cd3 t cell enriched fractions Search Results


mouse cd3 + t cell negative enrichment kit  (Becton Dickinson)
90
Becton Dickinson mouse cd3 + t cell negative enrichment kit
In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in <t>CD3</t> + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.
Mouse Cd3 + T Cell Negative Enrichment Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+t+cell+enriched+fractions/mouse+cd3+++t+cell+negative+enrichment+kit/pmc08811602-46-16-24
Average 90 stars, based on 1 article reviews
mouse cd3 + t cell negative enrichment kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
monocyte and cd3+ t cell subset enrichment columns  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc monocyte and cd3+ t cell subset enrichment columns
In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in <t>CD3</t> + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.
Monocyte And Cd3+ T Cell Subset Enrichment Columns, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+t+cell+enriched+fractions/monocyte+and+cd3++t+cell+subset+enrichment+columns/pm17239392-176-10-18
Average 90 stars, based on 1 article reviews
monocyte and cd3+ t cell subset enrichment columns - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
cd3+ t cell subset enrichment columns  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3+ t cell subset enrichment columns
In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in <t>CD3</t> + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.
Cd3+ T Cell Subset Enrichment Columns, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+t+cell+enriched+fractions/cd3++t+cell+subset+enrichment+columns/pm17239392-176-12-18
Average 90 stars, based on 1 article reviews
cd3+ t cell subset enrichment columns - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
cd3+t cell enrichment column  (Becton Dickinson)
90
Becton Dickinson cd3+t cell enrichment column
In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in <t>CD3</t> + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.
Cd3+T Cell Enrichment Column, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+t+cell+enriched+fractions/cd3+t+cell+enrichment+column/pm24368123-24-0-7
Average 90 stars, based on 1 article reviews
cd3+t cell enrichment column - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
human cd3 t cell large column enrichment kit  (Novus Biologicals)
N/A
   Buy from Supplier
rat cd3 t cell small column enrichment kit  (Novus Biologicals)
N/A
   Buy from Supplier
mouse cd3 t cell small column enrichment kit  (Novus Biologicals)
N/A
   Buy from Supplier
mouse cd3 t cell large column enrichment kit  (Novus Biologicals)
N/A
   Buy from Supplier
human cd3 t cell mini column enrichment kit  (Novus Biologicals)
N/A
   Buy from Supplier
mouse cd3 t cell mini column enrichment kit  (Novus Biologicals)
N/A
   Buy from Supplier

Image Search Results


In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in CD3 + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.

Journal: Journal for Immunotherapy of Cancer

Article Title: Delivery of CD47 blocker SIRPα-Fc by CAR-T cells enhances antitumor efficacy

doi: 10.1136/jitc-2021-003737

Figure Lengend Snippet: In vitro antitumor function of CAR-T cells. (A, B) The cytotoxic effect of CAR-T cells with different trop2-positive murine tumor cells. The expression of IFN-γ in CD3 + T cells when cocultured with MC38-Trop2 + cells (C), and 4T1-Trop2 + cells (D) were conducted by flow cytometry. For the in vitro phagocytosis assay, BMDMs were cocultured with CFSE-labeled MC38-Trop + cells at a 1:4 ratio in the supernatant of different T cells (E) or the medium of cocultured T cells with tumor cells (F). The cells were harvested after 4 hours coculture, and primary macrophages were identified by flow cytometry using anti-F4/80. Phagocytosis rate was determined as the percentage of CFSE + macrophages. (mean±SEM, n=3). Representative data from at least three independent experiments are shown. *p<0.05, **p<0.01, ****p<0.0001. BMDMs, bone marrow-derived macrophages; CAR, chimeric antigen receptor; CFSE, carboxyfluorescein diacetate succinimidyl ester; ns, not significant; UTD, untransduced.

Article Snippet: Mouse T cells isolated from wild-type C57BL/6 or BALB/c female mouse splenocytes were purified with a mouse CD3 + T cell negative enrichment kit (BD, USA).

Techniques: In Vitro, Expressing, Flow Cytometry, Phagocytosis Assay, Labeling, Derivative Assay

Secretion of SIRPα-Fc by CAR T cells modulate the immune cells in tumor-bearing mouse. Flow cytometry was used to detect the proportion of CAR + in tumor-bearing mouse spleen. (A) CAR positive rate in CD3 + T cells. (B) CAR positive rate in CD4 + T cells, (C) CAR positive rate in CD8 + T cells. (D) CD44 and CD62L of CD3 + CAR + T cells in mouse spleen. Flow cytometry analysis of (E) CD4 + CAR + T cells, (F) CD8 + CAR + T cells, (G) CD45 + CD11c + cells, and (H) CD11b + Ly6G + cells in tumor tissues. (I) Frequency of M1-like (CD206 — MHCII high ) and (J) M2-like (CD206 + MHC II low/neg ) TAMs cell populations in CD45 + CD3 — CD11b + F4/80 + in tumor tissues. All data are presented as mean±SEM from experiments, N=3 mice/group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CAR, chimeric antigen receptor; MHC, major histocompatibility complex; ns, not significant; SIRPα, signal regulatory protein α; TAM, tumor-associated macrophages; UTD, untransduced.

Journal: Journal for Immunotherapy of Cancer

Article Title: Delivery of CD47 blocker SIRPα-Fc by CAR-T cells enhances antitumor efficacy

doi: 10.1136/jitc-2021-003737

Figure Lengend Snippet: Secretion of SIRPα-Fc by CAR T cells modulate the immune cells in tumor-bearing mouse. Flow cytometry was used to detect the proportion of CAR + in tumor-bearing mouse spleen. (A) CAR positive rate in CD3 + T cells. (B) CAR positive rate in CD4 + T cells, (C) CAR positive rate in CD8 + T cells. (D) CD44 and CD62L of CD3 + CAR + T cells in mouse spleen. Flow cytometry analysis of (E) CD4 + CAR + T cells, (F) CD8 + CAR + T cells, (G) CD45 + CD11c + cells, and (H) CD11b + Ly6G + cells in tumor tissues. (I) Frequency of M1-like (CD206 — MHCII high ) and (J) M2-like (CD206 + MHC II low/neg ) TAMs cell populations in CD45 + CD3 — CD11b + F4/80 + in tumor tissues. All data are presented as mean±SEM from experiments, N=3 mice/group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. CAR, chimeric antigen receptor; MHC, major histocompatibility complex; ns, not significant; SIRPα, signal regulatory protein α; TAM, tumor-associated macrophages; UTD, untransduced.

Article Snippet: Mouse T cells isolated from wild-type C57BL/6 or BALB/c female mouse splenocytes were purified with a mouse CD3 + T cell negative enrichment kit (BD, USA).

Techniques: Flow Cytometry